Topic Chapter (pgs) {Problems}

1. Tetrad analysis 7(194-200) {10-15}
- ordered vs unordered
- first vs. second division segregation
- mapping

2. Anticipation, imprinting 7(184-186)

3. Recombination
- Mitotic recombination 5(136-138) {21-22)
- Holliday and DSB models 11(317-319) {26}
- gene conversion

4. Chromosome structure 17 {1-17}
- genome complexity
- supercoiling & linking number
- nucleosomes, heterochromatin, scaffold
- CEN & TEL
- exon shuffling
- repetitive DNA
- multigene & tandem repeat families
- genomics

5. Recombinant DNA 15 {1-21}
- clone screening, S & N blots Appendix 1-8
- restriction maps, dideoxy sequencing
- PCR, gene transfer
- genomics
- transgenics

6. Use of Recombinant DNA 16 {1-15}
- RFLP mapping
- positional cloning
- diagnostics
- gene therapy
- knock-out mice
- forensics
- genomics
- biotechnology

7. Mutation 14 {1-30}
- mutation rate
- random vs adaptation
- tautomerisation (transitions, transversions)
- chemical & UV mutagenesis
- human case studies
- DNA repair
- reverse genetics (M.Smith's Nobel prize)
- jumping genes & mutation Topic Chapter (pgs) Problems

8. Cytoplasmic inheritance 8 {1-16}
- maternal effect & maternal inheritance, pathology
- modified wobble
- genomics (mt DNA)
- D-loop region

9. Eukaryotic gene regulation 19 {1-10}
- transcriptional regulation
- promoters vs enhancers, TFs
- DNA-binding motifs
- transcriptional activation
- hormonal regulation
- DNA methylation, DNA amplification
- posttranscriptional regulation

10. Cancer genetics 21 {1-13}
- cell cycle & cancer
- genes that cause and suppress cancer
- proto-oncogenes > oncogenes
- a genetic model-colon cancer
- cytogenetics
- environmental influences

11. Immunogenetics 22 {1-13}
- the immune response
- antibody structure
- generation of Ig & TCR diversity
- major histocompatibility complex
- some human pathologies

12. Evolutionary genetics 25 {1-14}
- polymorphisms
- speciation
- the molecular clock
- molecular phylogeny
- the third chimpanzee?
- gene duplication & pseudogenes
- the classical vs neutralist hypothesis

13. Developmental genetics 20 {1-12}
- progressive differential transcription
- switch points
- restriction of cell fate
- selector and segmentation genes
- homeodomains