WELCOME TO:
Biology 420.3.1 - Plant Molecular Biology
Winter - 2005-06
Instructor: Dr. P.C. Bonham-Smith - Rm. 166 (office): Biol 127 (lab)
Phone 966-4457
email: peta.bonhams@usask.ca
web: http://homepage.usask.ca/~pcb512/
Lab: Ag 2D88
Ms. Donna Lindsay- Rm 127 Phone: 966-4430
Evaluation Percentage:
Term paper (Instructions)
- DUE: 4 pm Friday 24th February- 25%
Lab work and reports (Instructions)
- 25%
Oral presentation & participation (28th March & 4th April) - 20%
Final exam - 30%
- exams not written will be assigned zero unless a written reason is
received
PRESENTATION SCHEDULE
PREVIOUS FINAL EXAMS:
2001
2000
Course outline:
Lecture 1 - Assignment of Topics for term papers - Topics to be researched
and presented orally
1. Plant transformation (5 lectures)
- Agrobacterium
- Ballistics
- vacuums & dips
2. Plant Genome (3 lectures)
3. Transposable elements & transposon tagging (8 lectures)
- Transposable elements
- Ac-Ds
- MITES
- Retrotransposons
- Tagging
4. Plant Cell Cycle (6 lectures)
- Principles of cell cycle regulation
- Plant cell cycle regulators
- CDKs
- cyclins
- CDK inhibitors
- Cell division and plant development
5. Plant pathogen interactions - Dr. Pierre Fobert, PBI/NRC (3
lectures)
- Definition of terminology and the gene for gene hypothesis
- Genes involved in the defense response
- Genes involved in pathogenicity
- Structure of R(resistance)genes
- Signal transduction pathway(s)
6. The Chloroplast (6 lectures)
- Proplastid, chloroplast, chromoplast, amyloplast
- Regulation of differentiation of the proplastid
- Size and copy number, gene arrangement, codon usage
- RNA editing, trans-splicing
7. Homeotic gene interactions in flower development (5 lectures)
- Phyllotaxy and flower shape
- Classes of mutants (meristem identity vs. organ identity)
- A, B, C model
- MADS box genes, isolation, structure, expression...
- Others (leafy, ap2, ufo)
- Interactions amongst homeotic genes
8. Environmental Effects of Gene Expression (3 lectures)
LAB SCHEDULE
| LAB 1 |
10th JANUARY |
| BIOL 307 |
Part 1: Bioinformatics |
| Ag2D88 |
Part 2: Checking out primers |
| LAB 2 |
17th JANUARY |
| Ag2D88 |
|
| LAB 3 |
24th JANUARY - Lab report 1 due |
| Ag2D88 |
|
| LAB 4 |
31st JANUARY |
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| LAB 5 |
7th FEBRUARY |
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***SPRING BREAK –
13th-17th February*** |
| LAB 6 |
21st FEBRUARY |
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| LAB 7 |
28th FEBRUARY |
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| LAB 8 |
7th March |
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| LAB 9 |
14th March |
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| LAB 10 |
21st March |
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WORK ON LAB REPORT 2 |
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| LAB 11 & 12 |
28th March - 4th April |
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TERM PAPER PRESENTATIONS |