Wolf Coat Color Genetics

A brief review of the genes controlling wolf (Canis lupus) coat colors and patterns. Some of this research was conducted in our own University of Saskatchewan lab, often as a comparison with the dog. Some is from the research of others.

I've only seen wolves in the wild once, and that was just last winter in Saskatchewan. I didn't have a camera with me, and wasn't close enough to get a good photo anyway. This summer I saw this wolf track in the Spatsizi wilderness of northern British Columbia and could at least photograph it! Some of the wolves we've studied come from this area. All of the wolves we've studied are from Canada.


This webpage was mounted on October 4, 2014 and last updated on November 21, 2014 by Sheila Schmutz. sheila.schmutz@usask.ca


DNA Studies To Date

Wolves have a wide geographic range and also considerable coat color variation, although not as much as the dog.

Tanned wolf pelts hanging on a wall, from a display at historic Fort Edmonton. Photo, Sheila Schmutz.


ASIP

In a DNA study of the agouti signal protein (ASIP) in coyotes, wolves, and dogs we found that there was no difference between the wolf and dog, except for a few mutations that cause specific coat color patterns in the dog (fawn, black-and-tan, and recessive black). Wolves exhibit the pattern known as wolf sable, with banded hairs on the back, and some solid black hairs interspersed. Some dogs also exhibit this pattern.

The wolf in the photo at the right shows the classic ASIP wild type pattern, with dark tipped hairs. Photo by Ray Collingwood, Collingwood Brothers, used with permission.

  • Schmutz, S. M., T. G. Berryere, J. L. Barta, K.D. Reddick, and J. K. Schmutz 2007. Agouti sequence polymorphisms in coyotes, wolves and dogs suggest hybridization Journal of Heredity 98:351-355.

  • MC1R

    The photograph is a mounted specimen on display at Elk Ridge Resort, on the edge of Prince Albert National Park, Saskatchewan. I've seen wolf tracks in the fresh snow there.

    In 2013, Ollivier et al. of France published a very interesting study based on archaeological specimens of wolves and early dogs, and included some living dogs. They obtained the terminal portion of the MC1R gene from these samples. They describe the locations for two wolf samples as found "at Torgashinskaya cave ca. 15000 to ca. 12000 cal. BP in an Upper Palaeolithic context and at Ulug Depe ca. 5550– ca. 4000 cal. BP in a Bronze Age context". The former is in Siberia and the latter in Turkmenistan. Being archeological specimens, they can't know the coat color of either of these wolves. A third sequence was also contributed to GenBank. All three are identical.

  • Ollivier M, Tresset A, Hitte C, Petit C, Hughes S, Gillet B, Duffraisse M, Pionnier-Capitan M, Lagoutte L, Arbogast R-M, Balasescu A, Boroneant A, Mashkour M, Vigne J-D, Hänni C. 2013. Evidence of Coat Color Variation Sheds New Light onAncient Canids. PlosOne 8:e75110.


    DEFB103 (also called CBD103)

    Wolves feeding on a frozen lake in the Spatsizi Wilderness of northern British Columbia. Photo by Ray Collingwood, used with permission.

    The groups from Stanford and UCLA joined forces to uncover the genetic basis of black coat color in wolves (Anderson et al. 2009). They focussed on the wolves of Yellowstone park. The same 3 bp deletion that causes black in many domestic dog breeds, was found to cause black in the wolf pack there, and also from Canada. They suggest that this K allele originated in very ancient dogs and has entered the wolf population via interspecies crosses long ago.

    Ollivier et al. (2013) also studied the K locus in their archeological wolf samples. They detected one copy of the KB allele in a wolf sample from Ulag Deppe in Siberia. This allele was also found in 4 of their early dog samples.

  • Anderson TM, vonHoldt BM, Candille SI, Musiani M, Greco C, Stahler DR, Smith DW, Padhukasahasram B, Randi E, Leonard JA, Bustamante CD, Ostrander EA, Tang H, Wayne RK, Barsh GS. (2009) Molecular and evolutionary history of melanism in North American gray wolves. Science. 6:1339-43.


    MITF

    The collaborative group from Sweden and Massachusetts has done a detailed "functional genomics" study to try to determine how 4 polymorphisms in the promoter region of the MITF gene might affect the amount and placement of the white markings in dogs. They studied the DNA for the promoter region of this gene from 60 wolves from various parts of the world.

    Because they found all four polymorphisms in wolves, they suggest that none could be the sole cause of any type of white marking pattern in dogs. However, based on their Table S1, only a single Scandinavian wolf was homozygous for the Exon 1B polymorphism and none were homozygous for SNP 21. Eight of the wolves were homozygous for the SINE. The Length Polymorphism data were not listed for most wolves. Since piebald or random spotting is inherited as a recessive in most dog breeds, only homozygous wolves would be expected to have white spotting of this type.

  • Korberg IB, Sundstrom E, Meadows JRS, Pielberg GR, Gustafson U, Hedhammar A, Karlsson EK, Seddon J,6, Soderberg A, Vila C, Zhang X, Akesson M, Lindblad-Toh K,4, Andersson G, Andersson L (2014) A Simple Repeat Polymorphism in the MITF-M Promoter Is a Key Regulator of White Spotting in Dogs. PlosOne 9:e104363.

  • Arctic Wolf (Canis lupus arctos)

    This "white" wolf skin is part of a historic display at Fort Edmonton, in Edmonton, Alberta. The interpreter was kind enough to show the undercoat of this skin which is not white at all. The fur is so thick and creamy that one wouldn't expect to find banded agouti hairs beneath! Hence this is not strictly speaking, a "white" wolf.


    Links to Related Sites


    for further information contact:

    Sheila M. Schmutz, Ph.D., Professor

    Department of Animal and Poultry Science

    College of Agriculture and Bioresources

    University of Saskatchewan

    Saskatoon, Canada S7N 5A8

    e-mail sheila.schmutz@usask.ca